HotStart DNA Polymerase, 1000 U, 10 x rxn buffer with 20 mM MgCl2, 10 mM dNTPs from Bioneer, Inc.

Using exclusive enzyme-mediated method Bioneer's HotStart DNA polymerase completely releases all polymerase activity during the first denaturation step. At the temperature above 70°C, a thermostable pyrophosphatase initiates pyrophosphate hydrolysis and activates the DNA polymerase. This prevents the formation of non-specific products and primer-dimers during the reaction set-up process and results in improved PCR specificity